Synthesis of Complementary DNA

cDNA Synthesis Protocol by Reverse Transcription

Hey Friends, cDNA (complementary DNA) is synthesized from an RNA template. This is done by Reverse Transcription. In this example, the oligo d(T)-primers are used to prime the cDNA synthesis. An alternative would be to use random hexamers as primers. cDNA is synthesized before qPCR to check gene expression levels. qPCR: https://www.youtube.com ...

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